AN INVITRO STUDY ON EUGENIA JAMBOLANAPLANT EXTRACTIN ISOLATED RABBIT ILIUM SHOWING SPASMOLYTIC EFFECTS
Muhammad Naeem Riaz
1. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad. Pakistan.
2. Faculty of Pharmacy, University of Central Punjab, Lahore. Pakistan.
3. Dean, Faculty of pharmacy, University of Central Punjab, Lahore. Pakistan.
4. Department of Pharmacology, University of Health Sciences, Lahore. Pakistan.
Keywords: Jamun fruit, Eugenia jambolana, acetylcholine, histamine, serotonin, calcium induced contraction, isolated rabbit ileum
Gastrointestinal smooth muscle spasm is the major cause of symptoms of different gastrointestinal disorders. This study was undertaken to examine the effects of Jamun fruit (Eugenia jambolana) extract. The experiment was performed on forty rabbits, properly dissected. Study was performed on Power Lab System. Acetylcholine, histamine, serotonin, and calcium were used in increasing doses to obtain significant contractions in the ilium until the ceiling effect was observed. Thereafter, these drugs were used in the presence of different doses of Jamun extract. Shift of different concentration-response curves in each group was obtained. Percentage inhibition was determined in each group.
Statistical analysis showed that in the presence of antagonist dose response curve in acetylcholine and calcium group were more significant than serotonin and histamine, while low doses of serotonin and histamine showed initially spasmogenic effects. Muscle contraction was measured using isometric transducer. Difference in proportions was examined by using ANOVA. Before and after using the drugs, the changes were compared and significance of the study was judged. It can be concluded from current results that Jamun can provide useful spasmolytic effect in different intestinal disorders.