ANTIOXIDANT POTENTIAL AND TOTAL PHENOLIC CONTENT OF URTICA DIOICA (WHOLE PLANT)
Bhuwan Chandra Joshi, Minky Mukhija, Sushmita Semwal
Keywords: Urtica dioica Linn.(UD), DPPH assay, NO assay, Total phenolic content.
Whole plant of Urtica dioica Linn. (Urticaceae) were subjected to extraction with different solvent according to polarity and further to obtain antioxidant rich extract. Different concentrations of different solvent extracts were subjected to antioxidant assay by DPPH, Nitric oxide NO scavenging method and Total phenolic contents. The IC50 values for different solvent extracts (Petroleum ether, ethyl acetate, n-butanol, ethanol) of Urtica dioica Linn.(UD) were found to be 215.96 ± 0.066, 78.99 ± 0.171, 168.24 ± 0.346 and 302.90 ± 0.141 respectively in comparison to L-Ascorbic acid as standard with IC50 values of 26.24 ± 0.193 respectively in DPPH model. In nitric oxide radical scavenging activity the IC50 values were found to be 172.38 ± 0.635, 101.39 ± 0.306, 141.23 ± 0.809, 202.26 ± 0.67 and 55.38 ± 0.56 for different extracts and L-Ascorbic acid respectively. The highest Total phenolic content was found to be 13.06 ± 0.15 mg GAE/g in ethyl acetate extract. However, the ethyl acetate extract showed a better free radical scavenging activity as compared to other extracts.
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