MEASUREMENT OF CYP1A2 PHENOTYPE USING FEMALE VOLUNTEER PLASMA: A FOCUS ON CAFFEINE AND PARAXANTHINE AS A PROBE
Saima Rehman, Shagufta Kamal, Shumaila Kiran, Ismat Bibi, Iqbal Hussain
Department of Applied and Biochemistry, Government College University, Faisalabad-38000, Pakistan . Department of Chemistry, Islamia University, Bahawalpur 63100, Pakistan. Department of Botany, Government College University, Faisalabad-38000, Pakistan
Keywords: Cytochrome P-450, caffeine, metabolism, cancer, volunteers
Abstract

Caffeine neither causing didn't decrease the risk of cancer, yet it used just to note the activity of cytochrome P-4501A2 (CYP1A2) by converting into its metabolite i.e., paraxanthine. The purpose of the present study was to determine the caffeine and its metabolite phenotypes and their relation to cancer risk in healthy female volunteers of local population in Pakistan. The average value of metabolic ratio [(1,7-dimethylxanthine (17X) and Caffeine 1,3,7-trimethylxanthine (137X)] was found to be 1.182995 ± 0.21137. BMI (used to categorize into different groups, i.e., overweight, underweight etc.) of all volunteers were found to be 19.93Kg/m2. Retention time was 15 and 37 min for 1,7-dimethylxanthine (17DMX) and 1,3,7 trimethylxanthine (137TMX), respectively. The linearity of calibration curve of 137TMX and 17DMX were covered 0–12 µg/mL (R2 = 0.994). A significant positive correlation was observed between metabolic ratio and cancer risk factors. We could concluded that all the volunteers are fast metabolizers having a greater risk of cancerous diseases.

Article Information

Identifiers and Pagination:
Year:2016
Volume:8
First Page:34
Last Page:42
Publisher Id:19204159.8:2.2016
Article History:
Received:November 24, 2015
Accepted:January 7, 2016
Collection year:2015
First Published:April 1, 2016


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Prof. Dr. Cornelia M. Keck (Philipps-Universität Marburg)
Marburg, Germany

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Welcome to the research group of Prof. Dr. Cornelia M. Keck in Marburg. Cornelia M. Keck is a pharmacist and obtained her PhD in 2006 from the Freie Universität (FU) in Berlin. In 2009 she was appointed as Adjunct Professor for Pharmaceutical and Nutritional Nanotechnology at the University Putra Malaysia (UPM) and in 2011 she obtained her Venia legendi (Habilitation) at the Freie Universität Berlin and was appointed as a Professor for Pharmacology and Pharmaceutics at the University of Applied Sciences Kaiserslautern. Since 2016 she is Professor of Pharmaceutics and Biopharmaceutics at the Philipps-Universität Marburg. Her field of research is the development and characterization of innovative nanocarriers for improved delivery of poorly soluble actives for healthcare and cosmetics. Prof. Keck is executive board member of the German Association of Nanotechnology (Deutscher Verband Nanotechnologie), Vize-chairman of the unit „Dermocosmetics“ at the German Society of Dermopharmacy, active member in many pharmaceutical societies and member of the BfR Committee for Cosmetics at the Federal Institute for Risk Assessment (BfR).

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