Canadian Journal of Applied Sciences


ISSN: 1925-7430
Short Key Title: Can J App Sci
DOI: http://dx.doi.org/10.21065/19257430
Start Year: 2011

EFFECT OF ACIDIC AND ALKALINE MEDIUM ON FEXOFENADINE BRANDS USING UV SPECTROPHOTOMETER
Safila Naveed
Faculty of Pharmacy Jinnah University for women Karachi, Pakistan.
Keywords: Fexofenadine,acidic medium, alkaline medium, U.V spectrophotometer.
Abstract

Fexofenadine is widely used drug for allergic conditions especially Rhiniti and is a selective histamine H1 receptor antagonist. In our recent research we study the effect of acidic and alkaline medium on four different brands of Fexofenadine, for this the solutions of different brands of Fexofenadine were subjected to neutral,alkaline and acidic medium. When Fexofenadine brands subjected to 0.1 N HCl and 0.1N NaOH for 30 minutes, Fexofenadine brands showed variable availability results. Xanidine and Telefast donot show any change in absorbance in acidic medium but shows a decrease in absorbance in alkaline medium. While Allerga shows a decrease in absorbance in both acidic and alkaline medium. Fexet also shows a decrease in absorbance in acidic medium but shows a increase in absorbance in alkaline medium.

Article Information

Identifiers and Pagination:
Year:2015
Volume:5
First Page:1
Last Page:4
Publisher Id:CanJAppSci (2015 ). 5. 1-4
Article History:
Received:November 03, 2014
Accepted:March 21, 2015
Collection year:2014
First Published:January 1, 2015

INTRODUCTION

Chemically Fexofenadine is((±)-2-[4-[1-hydroxy-4-[4- (hydroxydiphenylmethyl) piperidino] butyl]phenyl] - 2-methylpropanoic acid and is a selective histamine H1 receptor antagonist.it is one of the most used seasonal allergic rhinitis and chronic urticaria treatments [1]. Fexofenadine belongs to antihistamine class of drug and it is  used in the treatment of allergy symptoms, such as nasal congestion , hay fever and urticaria.[2] It is often said to be a third-generation antihistamine. Its action is limited to outside the brain and spinal cord. fexofenadine produces a minimal sedation. [3] The safety profile of fexofenadine is quite favorable, it shows no cardiovascular or sedative effects to occur even when taking ten times the recommended dose.[4] the half-life of the drug is shorter than cetirizine, so it often must be taken twice daily.[5] Moreover cetirizine causes more sleepiness than fexofenadine.[6]  Fexofenadine is infact a racemic mixture of R- and S-enantiomers. Both enantiomers shows equal potency in their clinical effects [7]

Figure-1 Structure of Fexofenadine

EXPERIMENTAL METHODOLOGY

Material and reagents

Pyrex glass wares, beakers, measuring volumetric flask, cylinder pipette, funnel and stirrer were used. All glass wares were washed and rinsed with double distilled water . Reagents used were as follows 0.1N Sodium hydroxide, 0.1N Hydrochloric acid and de-ionized water or double distilled water. All the Reagents were of Analytical grade.

Instruments

UV Lamp Power of 8N, Serial NO: N 045571, LF-204.LS ‘4W-254 and 365 nm’, Spectrophotometer with a quartz cuvette T80 UV-VI spectrometer ‘PG Instrument’, Weighing Balance Item PA214C: ‘Pioneer OHAIUS’ and Water Bath ‘HH-4’ having digital and constant temperature tank. 

Preparation of 0.1 N Hydrochloric acid and Sodium hydroxide

4 grams of sodium hydroxide was dissolved in small amount of water and transferred in 100ml volumetric flask and volume was made up to mark with de-ionized water. 

8.3ml analytical grade hydrochloric acid having 37% purity and 12N normality was taken  in a volumetric flask and volume was made up to the mark with DI water.

Preparation of solution of different brands of Fexofenadine

each tablet individually was weighed on the weighing balance. the tablets individually were grounded and triturated with the help of mortar and pestle to make them in powder form. the same procedure was repeated for the tablets of each brand. For the preparation of 200ppm solution of each brand accurately weighed triturated powder of each brand i.e. Xadine, Telfast, Allerga, Fexet equivalent to 20 mg of Fexofenadine transfrsed in to a beaker and dissolved in small quantity of DI water. these primary solutions were transfered separately into six volumetric flasks of 100ml. Finally volume was made-up with de-ionized water. The absorbance of solutions of each brand of fexofenadine was determined by using UV-Visible spectrophotometer, at wavelength max of 220nm.

Procedure:

To determine the effect of acid and base on Fexofenadine, 5 ml of 200 ppm solution of each brand of Fexofenadine was transferred in to two separate test tubes then 5 ml of 0.1 N hydrochloric acid HCl was added in one test tube and 5 ml of 0.1 N sodium hydroxide NaOH was added in another test tube respectively. Then the tubes were left for 30 minutes. The absorbance of the solution was determined using spectrophotometer at wavelength max 220nm.

 

 

 

Table-1 Absorbance of different brands of Fexofenadine

Absorbance of different brands of Fexofenadine

Parameters

Xanidine

Telefast

Allerga

Fexet

water

2.154

2.254

2.98

2.382

Acid

2.154

2.254

2.313

2.365

Base

2.032

2.332

2.493

2.572

 

Figure-2 Absorbance patteren of different brands of Fexofenadine in different medium

 

RESULT AND DISCUSSION

The 200ppm solution of Four different brands of fexofendine Xadine, Telfast, Allerga, Fexet were subjected to different medium for thirty minutes i.e acidic and alkaline medium ,their absorbance was determined at wavelength maximum which was found to be 220nm. Xanidine and Telefast donot show any change in absorbance in acidic medium but shows a decrease in absorbance in alkaline medium. At neutral PH Xanidine shows a absobance of 2.154, in basic medium it show absorbance of 2.032 and in acidic medium it shows  the same absorbance as in neutral medium i.e 2.154.while at neutral PH Telefast shows a absobance of 2.254, in basic medium it show absorbance of 2.332 and in acidic medium it shows  the same absorbance as in neutral medium i.e 2.254.While Allerga shows a decrease in absorbance in both acidic and alkaline medium, in water i.e at neutral PH it shows a absobance of 2.98, in basic medium it show absorbance of 2.493 and in acidic medium it shows absorbance of 2.313. Fexet also shows a decrease in absorbance in acidic medium but shows a increase in absorbance in  alkaline medium, in water i.e at neutral PH it shows a absobance of 2.382, in basic medium it show absorbance of 2.572 and in acidic medium it shows absorbance of 2.365.

 

REFERENCES

1.      Kim RB. Transporters and xenobiotic disposition. Toxicology. 2002;181(182):291–7. 

2.      Bachert, C (May 2009). "A review of the efficacy of desloratadine, fexofenadine, and levocetirizine in the treatment of nasal congestion in patients with allergic rhinitis"Clin Ther 31 (5): 921–44. doi:10.1016/j.clinthera.2009.05.017PMID 19539095. Retrieved18 April 2014.

3.       Compalati, E; Baena-Cagnani, R; Penagos, M; Badellino, H; Braido, F; Gómez, RM; Canonica, GW; Baena-Cagnani, CE (2011). "Systematic review on the efficacy of fexofenadine in seasonal allergic rhinitis: a meta-analysis of randomized, double-blind, placebo-controlled clinical trials.". International Archives of Allergy and Immunology 156(1): 1–15. doi:10.115

4.      Philpot, EE (Jan–Feb 2000). "Safety of second generation antihistamines". Allergy Asthma Proc 21 (1): 15–20. 

  1. Church, Martin; Church, Diana (2013). "Pharmacology of antihistamines"Indian Journal of Dermatology 58 (3): 219–224. doi:10.1097/WOX.0b013e3181f385d9.PMC 3666185PMID 23282332. Retrieved 18 April 2014.
  2. Tashiro, M; Sakurada Y, Iwabuchi K, Mochizuki H, Kato M, Aoki M, Funaki Y, Itoh M, Iwata R, Wong DF, Yanai K (Aug 2004). "Central effects of fexofenadine and cetirizine: measurement of psychomotor performance, subjective sleepiness, and brain histamine H1-receptor occupancy using 11C-doxepin positron emission tomography."J Clin Pharmacol 44 (8): 890–900. doi:10.1177/0091270004267590PMID 15286093. Retrieved 18 April 2014.

7.     Robbins DK, Castles MA, Pack DJ, Bhargava VO, Weir SJ. Dose proportionality and comparison of single and multiple dose pharmacokinetics of fexofenadine (MDL 16455) and its enantiomers in healthy male volunteers.Biopharm Drug Dispos. 1998;19:455–63.


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Dr. Xianghui Qi is working as Professor in the School of Food & Biological Engineering, Jiangsu University, China. His research interests: Biosynthesis of high value-added chemicals by microbes and engineered strains; Discovery of novel genes, enzymes and new strains; Rational & Irrational design of microbial enzymes; Isolation, identification and evolution of microbes; Metabolic engineering & Pathway engineering of functional microbes, and biotransformation; Metabolic regulation based on the research of microbial omics; Application of high value-added products including functional sugar alcohols by biosynthesis and biotransformation based on microbial engineered strains.

Journal Highlights
Abbreviation: Can J Appl Sci
doi: http://dx.doi.org/10.21065/19257430
Frequency: Annual
Current Volume: 7 (2017)
Next scheduled volume: December, 2018  
Back volumes: 1-7
Starting year: 2011
Nature: Online
Submission: Online
Language: English

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