is((±)-2-[4-[1-hydroxy-4-[4- (hydroxydiphenylmethyl) piperidino] butyl]phenyl]
- 2-methylpropanoic acid and is a selective histamine H1 receptor antagonist.it
is one of the most used seasonal allergic rhinitis and chronic urticaria
treatments . Fexofenadine belongs to antihistamine
class of drug
and it is used in the treatment of allergy symptoms, such as
nasal congestion , hay fever and urticaria. It
is often said to be a third-generation
antihistamine. Its action
is limited to outside the brain and spinal cord. fexofenadine produces a
minimal sedation.  The safety profile of fexofenadine is quite
favorable, it shows no cardiovascular or sedative effects to occur even when
taking ten times the recommended dose. the half-life of the drug is shorter than cetirizine, so it often must be taken twice daily. Moreover
cetirizine causes more sleepiness than fexofenadine. Fexofenadine is infact a racemic
mixture of R- and S-enantiomers.
Both enantiomers shows equal potency in their clinical effects 
Figure-1 Structure of
Pyrex glass wares, beakers, measuring volumetric flask, cylinder
pipette, funnel and stirrer were used. All glass wares were washed and rinsed
with double distilled water . Reagents used were as follows 0.1N Sodium
hydroxide, 0.1N Hydrochloric acid and de-ionized water or double distilled
water. All the Reagents were of Analytical grade.
UV Lamp Power of 8N, Serial NO: N 045571, LF-204.LS ‘4W-254 and
365 nm’, Spectrophotometer with a quartz cuvette T80 UV-VI spectrometer ‘PG
Instrument’, Weighing Balance Item PA214C: ‘Pioneer OHAIUS’ and Water Bath
‘HH-4’ having digital and constant temperature tank.
4 grams of sodium hydroxide was dissolved in small amount of water
and transferred in 100ml volumetric flask and volume was made up to mark with
8.3ml analytical grade hydrochloric acid having 37% purity and 12N
normality was taken in a volumetric flask and volume was made up to the
mark with DI water.
individually was weighed on the weighing balance. the tablets individually were
grounded and triturated with the help of mortar and pestle to make them in
powder form. the same procedure was repeated for the tablets of each brand. For
the preparation of 200ppm solution of each brand accurately weighed triturated powder of each brand i.e.
Xadine, Telfast, Allerga, Fexet equivalent to 20 mg of Fexofenadine transfrsed in to a beaker and dissolved in small
quantity of DI water. these primary solutions were transfered separately into six
volumetric flasks of 100ml. Finally volume was made-up with de-ionized water. The absorbance of solutions
of each brand of fexofenadine was determined by using UV-Visible
spectrophotometer, at wavelength max of 220nm.
To determine the effect of acid and base on Fexofenadine, 5 ml of
200 ppm solution of each brand of Fexofenadine was transferred in to two
separate test tubes then 5 ml of 0.1 N hydrochloric acid HCl was added in one
test tube and 5 ml of 0.1 N sodium hydroxide NaOH was added in another test
tube respectively. Then the tubes were left for 30 minutes. The absorbance of
the solution was determined using spectrophotometer at wavelength max 220nm.
Table-1 Absorbance of
different brands of Fexofenadine
different brands of Fexofenadine
Absorbance patteren of different brands of Fexofenadine in different medium
RESULT AND DISCUSSION
The 200ppm solution of Four different brands of fexofendine Xadine, Telfast, Allerga, Fexet were subjected to different medium for
thirty minutes i.e acidic and alkaline medium ,their absorbance was determined
at wavelength maximum which was found to be 220nm. Xanidine and Telefast donot
show any change in absorbance in acidic medium but shows a decrease in
absorbance in alkaline medium. At neutral PH Xanidine shows a absobance of
2.154, in basic medium it show absorbance of 2.032 and in acidic medium it
shows the same absorbance as in neutral
medium i.e 2.154.while at neutral PH Telefast shows a absobance of 2.254, in
basic medium it show absorbance of 2.332 and in acidic medium it shows the same absorbance as in neutral medium i.e
2.254.While Allerga shows a decrease in absorbance in both acidic and alkaline
medium, in water i.e at neutral PH it shows a absobance of 2.98, in basic
medium it show absorbance of 2.493 and in acidic medium it shows absorbance of
2.313. Fexet also shows a decrease in absorbance in acidic medium but shows a
increase in absorbance in alkaline
medium, in water i.e at neutral PH it shows a absobance of 2.382, in basic
medium it show absorbance of 2.572 and in acidic medium it shows absorbance of
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