Compounds identified through GC-MS analysis
On the basis of NIST library major five phyto-constituents were
identified in the AL-extract, one in AS-extract, eight in ML-extract, and six
in MS-extract at different retention time (Table 3) and the total ion graphs
are presented in Figure (2 and 3). Benzene, 3-eicosyne, phytol, squalene in
AL-extract, bis (2 ethyl hexyle phthalate) in AS-extract, benzoic acid
4-methoxy-methyl ester, cyclopenta pyran-4-carboxylic acid, propenoic acid,
phytol, 2(4)-benzofuranone in ML-extract were identified at different retention
time followed by and 2(4H)-benzofuranone, benzyl benzoate, hexadecanoic acid,
9,12-octadecadienoic acid, phytol in MS-extract.
GC-MS analysis reports the similar group of compounds like methyl ester,
phytol, benzoic acid and cyclopenta pyran-4-carboxylic acid, hexadecanoic acid,
etc. accept benzyle benzoate, ecosyne, propanoic acid, squalene
2(4H)-benzofuranone. The compounds are used as additives for any kind of
preservation because they contain microbicidal agents. A new iridoid diglucoside, lupulinoside and eight known iridoid
glucosides, acetyl barlerin, ipolamiidoside,6-O-acetylshanzhiside methyl ester,
barlerin, shanzhiside methyl ester, mussaenosidic acid, 8-O-acetylshanzhiside,
and shanzhiside that have beenisolated from the flowers of B. lupulina .
Some of the constituents such as
phenol,2,4,bis (1,1-dimethylethyl)-tetradecanoic acid, 12- methyl-, methyl
ester, hexadecanoic acid, methyl ester, phytol and octadecanoic acid, have
proven antimicrobial potential [14-15]. Presence of cyclobutane, 1,1-dimethyl- 2-octyl, 2-hexyl-1-octanol, 1,
2-henzenedicarboxylic acid, mono (2-ethylhexyl) ester and 1-hentetracontanol
have been reported in B. lupulina
. The constituents contain
antimicrobial efficacy itself and lend support to the claim that these
phyto-constituents identified through HPTLC screening and GC-MS analysis may
attribute to the antibacterial property of B.
On the basis
of above results it may be concluded that the leaf and stem extracts in
different solvents showed significant antibacterial activity against all
selected pathogens. GC-MS analysis of B.
lupulina extracts identified a total of 20 phyto-constituents which may the
responsible for antibacterial activity.
ACKNOWLEDGMENTS: The authors
wish to thank the Head, Department of Botany and Microbiology, Gurukul Kangri
Vishwavidyalaya, Haridwar for providing laboratory facilities and Acharya
Balkrishna (Patanjali Yog Peeth, Haridwar) for HPTLC and GC-MS analysis.
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