MEASUREMENT OF CYP1A2 PHENOTYPE USING FEMALE VOLUNTEER PLASMA: A FOCUS ON CAFFEINE AND PARAXANTHINE AS A PROBE
Saima Rehman, Shagufta Kamal, Shumaila Kiran, Ismat Bibi, Iqbal Hussain
Department of Applied and Biochemistry, Government College University, Faisalabad-38000, Pakistan . Department of Chemistry, Islamia University, Bahawalpur 63100, Pakistan. Department of Botany, Government College University, Faisalabad-38000, Pakistan
Keywords: Cytochrome P-450, caffeine, metabolism, cancer, volunteers
Caffeine neither causing didn't decrease the risk of cancer, yet it used just to note the activity of cytochrome P-4501A2 (CYP1A2) by converting into its metabolite i.e., paraxanthine. The purpose of the present study was to determine the caffeine and its metabolite phenotypes and their relation to cancer risk in healthy female volunteers of local population in Pakistan. The average value of metabolic ratio [(1,7-dimethylxanthine (17X) and Caffeine 1,3,7-trimethylxanthine (137X)] was found to be 1.182995 ± 0.21137. BMI (used to categorize into different groups, i.e., overweight, underweight etc.) of all volunteers were found to be 19.93Kg/m2. Retention time was 15 and 37 min for 1,7-dimethylxanthine (17DMX) and 1,3,7 trimethylxanthine (137TMX), respectively. The linearity of calibration curve of 137TMX and 17DMX were covered 0–12 µg/mL (R2 = 0.994). A significant positive correlation was observed between metabolic ratio and cancer risk factors. We could concluded that all the volunteers are fast metabolizers having a greater risk of cancerous diseases.