antioxidants that are present in plants are responsible for inhibiting or
preventing the harmful consequences of oxidative stress. Plants contains free
radical scavengers like polyphenols, flavonoids and phenolic compounds .
These compounds have shown to have antioxidant activity. In the present paper,
we have evaluated the free radical scavenger activity of different extracts of Zanthoxylum alatum stem bark.
are number of assay designed to measure overall antioxidant activity/reducing
potential, as an indication of total capacity of plants to withstand free
radical stress .
is very convenient for the screening of number of samples of different
polarity. The measurement of the scavenging of DPPH radical allows to determine
the intrinsic ability of substance to donate hydrogen atom or electrons to this
reactive species in a homogenous system. Methanolic DPPH solution get reduced
because of the presence of antioxidant substances having hydrogen-donating
groups such as phenols and flavonoid compounds due to the formation of non
radical DPPH-H form .
Nitric oxide is a diffusible free radical that plays
an important role as an effectors molecule in diverse biological systems
including neuronal messenger, vasodilatation and antitumor activities .
Although, NO is involved in host defence, over production of these radical
contributes to the pathogenesis of various diseases . The petroleum ether
extract and ethyl acetate extract significantly inhibited NO in a dose
dependent manner with the IC50 being 72.39±1.53 and 94.81±2.56 µg/ml as compared with the
standards ascorbic acid having the IC50 values of 42.8±2.61 µg/ml.
The results indicated that both petroleum ether and ethyl acetate extract
contain the compounds which are able to inhibit NO and act as an antioxidant.
(III) reduction is often used as an indicator of electron-donating activity,
which is an important mechanism of phenolic antioxidant action . In the
reducing power assay, the presence of antioxidants in the samples would result
in the reducing of Fe3+ to Fe2+ by donating an electron
. Being good electron donors, phenolic compounds shows the reducing power
and have ability to convert ferric ion to ferrous ion by donating an electron
(20). Increasing absorbance at 700 nm indicates an increase in reductive
study, the different extracts of ZA showed high total antioxidant and DPPH
activity, NO and ferrous iron reducing capacities. Considerable correlations
were observed between DPPH and NO scavenging, reducing power and total phenols.
Antioxidant capacity may be associated with a high phenol content as Mansouri
et al. 2005 reported that most of the antioxidant activity of plants is derived
from phenols . Structurally, phenols comprise an aromatic ring having one
or more hydroxyl groups. The antioxidant activity of this type of molecule is
due to their ability to scavenge free radicals, donate hydrogen atoms or
electrons or chelate metal cations . Previously stem bark of ZA has been reported
to contain phenolic compounds .
present study, the antioxidant activity of petroleum ether and ethyl acetate
extracts may be attributed to the combined effects of the phenolic compounds
and the results are in full agreement with previous studies on other plants
all the extracts, petroleum ether and ethyl acetate extracts of ZA showed
potent antioxidant potential as determined by different procedures. Presence of
phenolic compounds in the extracts confirmed their utility as potent
express our sincere thanks to Punjab Technical University, Kapurthala for
allowing us to proceed with the research proposal. We also express our thanks
to the Management and Shri. Parveen Garg, Honorable Chairman, ISF College of
Pharmacy, Moga (Punjab) for providing necessary facilities.
1. Wiseman, H. and Halliwell, B. (1996). Damage to DNA by
reactive oxygen and nitrogen species: role in inflammatory disease and
progression to cancer. Biochemical
2. Sreeramulu, D., Reddy, C.V.K., Chauhan, A., Balakrishna, N.
and Raghunath, M. (2013). Natural Antioxidant Activity of Commonly Consumed
Plant Foods in India: Effect of Domestic Processing. Oxidative Medicine and Cellular Longevity Article ID 369479, 12
3. Reuter, S., Gupta, S.C, Chaturvedi, M.M. and Aggarwal, B.B.
(2010). Oxidative Stress, Inflammation, and Cancer: How are They Linked? Free Radical Biology & Medicine
4. Duracková, Z. Some Current Insights into Oxidative Stress.
(2010). Physiological Research
5. Katalinic, V., Milos, M., Kulisic, T. and Jukic, M. (2006).
Screening of 70 medicinal plant extracts for antioxidant capacity and total
phenols. Food Chemistry 94:550–557.
6. Yildrim, A., Oktay,
M. and Bilaloglu, V. (2001).
The antioxidant activity
of leaves of Cydonia vulgaris. Turkish Journal of Medical Science 31:23-27.
7. Halliwell, B. and Guteridge, J.M.C. (1989). Free Radicals in
Biology and Medicine. 2nd ed. Clarendon Press: Oxford, UK. pp. 1-20.
8. Waterman P.G., and Mole, S. (1994). Analysis of Phenolic
Plant Metabolites. Blackwell Cientific Publications, UK. pp. 85-87.
9. Gupta, A.K., Tandon, N. and Sharma, M. (2006). Zanthoxylum armatum. In: Quality
Standards of Indian Medicinal Plants Indian Council of Medical Research (ICMR).
Indraprasthra press, New Delhi. pp. 271-278.
10. Nadkarni, A.M. (2002). Indian Material Medica. Vol. 1.
Bombay Popular Prakashan, Mumbai. pp. 1302.
11. Kapoor, L.D. (1990). Handbook of Ayurvedic medicinal plants.
1st ed. CRC Press, Boca Raton. pp. 416.
12. Baquar, S.R.. (1989). Medicinal and poisonous plants of
Pakistan, 1st ed. Printas, Karachi, Pakistan. pp. 508.
13. Kumar, S. and Muller, K. (1999). Inhibition of keratinocyte
growth by different Nepalese Zanthoxylum species. Phytotherapy Research 13:214-217.
14. Mehta, M.B., Kharya, M.D., Srivastava, R. and Verma, K.C.
(1981). Antimicrobial and anthelmintic activities of the essential oil of Zanthoxylum alatum Roxb. Indian Perfumary 25:19-21.
15. Bhatt, N. and Upadhyaya, K. (2010). Anti inflammatory
activity of ethanolic extract of bark of Zanthoxylum
armatum D.C. Pharmacology Online
16. Ranawat, L., Bhatt, J. and Patel, J. (2010).
Hepatoprotective activity of ethanolic extracts of bark of Zanthoxylum armatum DC in CCl4 induced hepatic damage in
rats. Journal of Ethnopharmacology
17. Verma, N. and Khosa, R.L. (2010). Hepatoprotective activity
of leaves of Zanthoxylum armatum DC
in CCl4 produced hepatotoxicity in rats. Indian Journal of Biochemistry and Biophysics 47:124-127.
18. Tiwary, M., Naika, S.N., Tewary, D.K., Mittal, P.K. and
Yadav, S. (2007). Chemical composition and larvicidal activities of the
essential oil of Zanthoxylum armatum
DC (Rutaceae) against three mosquito vectors. Journal of Vector Borne Disease 44:198-204.
19. Gilani, S.N., Khan, A.U. and Gilani, A.H. (2010).
Pharmacological basis for the medicinal use of Zanthoxylum armatum in gut, airways and cardiovascular disorders. Phytotherapy Research 24:553-558.
20. Kar, A. and Borthakur, S.K. (2008). Medicinal plants used
against dysentery, diarrhea and cholera by the tribes of erstwhile Kameng
district of Arunachal Pradesh. Natural
Product Radiance 7:176-181.
21. Ramanujam, S.N. and Ratha, B.K. (2008). Effect of alcohol
extract of a natural piscicide fruits of Zanthoxylum
armatum DC on Mg2+- and Na+, K+ -ATPase
activity in various tissues of a freshwater air-breathing fish, Heteropneustes
fossilis. Aquaculture 283:77
22. Kumar, S., Singh, S.K., Ghildiyal, J.C., Baslas, R.K. and
Saxena, A.K. (2003). The lousicidal potential of the seed extract of Zanthoxylum alatum. Indian Veternary Journal 80:848-850.
23. Barkatullah, I.M. and Muhammad, N. (2011). Evaluation of Zanthoxylum armatum DC for in-vitro and in-vivo pharmacological screening. African Journal of Pharmacy and Pharmacology 5:1718-1723.
24. Harborne, J.B. (1998).
Phytochemical methods. 3rd
ed. Chapman and Hall, London. pp. 19-134.
25. Blois MS. (1958). Antioxidant determinations by the use of a
stable free radical. Nature
26. Garrat DC. (1964). The Quantitative Analysis of Drugs.
Chapman and Hall. pp. 456.
27. Oyaizu, M. (1986). Studies on products on browning reaction
prepared from glucose amine. Japanese
Journal of Nutrition 44:307-315.
28. Singleton, V.L. and Rossi, J.A. (1965). Colorimetry of total
phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology Viticulture
29. Khalaf, N.A., Shakya. A.K., Al-Othman, A, El-Agbar, Z. and
Farah, H. (2008) Antioxidant Activity of Some Common Plants. Turkish Journal of Biology 32:51-55.
30. Lugasi, A., Horvahovich, P. and Dworschak, E. (1999).
Additional Information to the in vitro
Antioxidant Activity of Ginkgo biloba
L. Phytotherapy Research
31. Mensour, L.L., Menezes,
F.S., Leitao, G.G., Reis A.S, Dos
Santos, T.C. and Coube, C.S. (2011). Screening of Brazilian Plant Extracts for
Antioxidant Activity by Use
of DPPH Free
Radical Method. Phytotherapy
32. Hagerman, A.E., Riedel, K.M., Jones, G.A., Sovik, K.N.
Ritchard, N.T. Hartzfeld P.W. Riechel, T.L., (1998): High molecular weight
plant polyphenolics (tannins) as biological antioxidants. Journal of Agriculture and Food Chemistry 46: 1887-92.
33. Guo, L., Fatig, R.O., Orr, G.L., Schafer, B.W., Strickland,
J.A. and Sukhapinda, K. (1999). Photorhabdus luminescens W-14
insecticidal activity consists of at least two similar but distinct
proteins. Indian Journal of Experimental Biology. 274:9836-9842.
34. Nabavi, S.M., Ebrahimzadeh, M.A., Nabavi, S.F., Fazelian, M.
and Eslami, B. (2009). In vitro
antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus
boissieriana growing in Iran. Pharmacognosy
35. Ebrahimzadeh, M.A., Nabavi, S.M., Nabavi, S.F., Bahramian,
F. and Bekhradnia, A.R. (2010). Antioxidant and free radical scavenging
activity of H. officinalis, Var. angustifolius, V. odorata, B. hyrcana
and C. speciosum. Pakistan Journal of
Pharmaceutical Sciences 23(1):29-34.
36. Sravani, T. and Paarakh, P.M. (2012). Antioxidant activity
of Hedychium spicatum Buch.-Ham.
Rhizomes. Indian Journal of Natural
Products and Resources 3(3):354-58.
37. Mansouri, A., Embared, G., Kokkalou, E. and Kefalas, P.
(2005). Phenolic profile and
antioxidant activity of
the Algerian ripe date
palm fruit (Phoenix dactylifera). Food Chemistry. 89:411-420.
38. Amarowicz, R., Pegg, R.B., Rahimi, M., Barl, B. and Weil,
J.A. (2004). Free radical
scavenging capacity and
antioxidant activity of
selected plant species from
the Canadian prairies,
Food Chemistry. 84:551–562.
39. Mukhija, M., Dhar, K.L. and Kalia, A.N. (2014). Bioactive
Lignans from Zanthoxylum alatum Roxb.
stem bark with cytotoxic potential. Journal
of Ethnopharmacology 152:106-112.
40. Mazandarani, M., Moghaddam, Z., Zolfaghari, M.R., Ghaemi,
E.A. and Bayat, H. (2012). Effects of Solvent Type on Phenolics and Flavonoids
Content and Antioxidant Activities in Onosma
dichroanthum Boiss. Journal of
Medicinal Plants Research. 6:4481-4488.
41. Kukic, J., Petrovic, S. and Niketic, M. (2006).
Antioxidant activity of four endemic Stachys
taxa. Biological and Pharmaceutical