EFFECT OF INDIGENOUS VARIETY OF ALOE VERA ON BACTERIOCIN PRODUCTION BY PROBIOTICS CULTURE
University Institute of Diet & Nutritional Sciences, The University of Lahore, Islamabad Campus, Pakistan
Keywords: Aloe vera, Probiotics, Zones of Inhibition, Anti Microbial Properties
Background: Aloe vera has long been used as a medicinal plant in the preparation of various gels, lotions and ointments besides its use in various food products.
Methodology: The present study was carried out at the Plants Genetics Research Institute, National Agricultural Research Centre, Islamabad. For the purpose of this study, an indigenous variety of Aloe vera was taken along with probiotics culture of Lactobacillus acidophilus, Lactococcus lactis and Lactobacillus helveticus. Aloe vera gel matrix was separated from outer cortex of leaves and media were prepared for the growth of respective probiotics cultures. Aloe vera was used in each probiotic culture against two pathogenic strains, i.e E. coli and S. aureus. In one plate, tryptone was replaced by 1% Aloe vera extract, while 0.5% and 1% Aloe vera extracts were added to other two plates. All the three probiotic cultures were separately inoculated in test tubes, which were incubated at 370C for 24 hours.
Results: After incubation of 24 hours, plates were observed on the next day for the measurement of inhibition zones. Strong to medium zones of inhibition were formed against E. coli and S. aureus for all three probiotic cultures when 1% Aloe vera extract was used.
Conclusions: This study revealed that the indigenous variety of Aloe vera exhibited significant anti microbial properties.
© 2016 The Author(s). This open access article is distributed under a Creative Commons Attribution (CC-BY) 4.0 license.
You are free to:
Share — copy and redistribute the material in any medium or format
Adapt — remix, transform, and build upon the material for any purpose, even commercially.
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made.
You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
No additional restrictions
You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits