Advanced Food and Nutritional Sciences


ISSN: -
Short Key Title: Adv Food Nutri Sci
DOI: http://dx.doi.org/10.21065/
Start Year: 2016

CLONING AND EXPRESSION OF LIPASE GENE IN E. COLI
Zeeshan Nadeem, Muhammad Noman Ishaq, Muhammad Shoaib, Dr. Amin Athar
Institute of Biochemistry & Biotechnology, University of the Punjab, Lahore, Pakistan
Keywords: Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa.), IPTG, SDS-PAGE
Abstract

After carbohydrates and proteases, based on total sales volumes, lipases are measured to be the third largest group. Lipases have particular significance in biotech industry because of numerous applications in the food industry, medicine industry, enzymatic production of lipophilic chemicals and in production of biological detergents. Lipases are mostly produced commercially by heterologous expression system. In this study, gene of extracellular lipase of Pseudomonas aeruginosa has been expressed in E. coli. The gene consists of 1700 bp. By designing gene specific primers, 1700 bp lipase gene was amplified from the genomic DNA of P. aeruginosa. The Pseudomonas aeruginosa lipase (PAL) gene was cloned in pTZ57R/T and transformed.in E. coli DH5a. Positive clones carrying pTZ-PAL were initially screened by blue white screening and further confirmed by colony PCR, plasmid PCR and restriction analysis. For expression of recombinant protein, the restricted gene was subcloned in pET28a (+) expression. vector and introduced in E. coli strain BL21. Upon IPTG induction, the expression of recombinant lipase was analysed by SDS-PAGE. Maximum expression was observed after 14 hours of 0.8mM IPTG induction at 37°C.

Article Information

Identifiers and Pagination:
Year:2019
Volume:4
First Page:20
Last Page:20
Publisher Id: (2019 ). 4. 20-20
Article History:
Received:June 15, 2019
Accepted:June 30, 2019
Collection year:2019
First Published:July 23, 2019


© 2016 The Author(s). This open access article is distributed under a Creative Commons Attribution (CC-BY) 4.0 license. You are free to: Share — copy and redistribute the material in any medium or format Adapt — remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. No additional restrictions You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits
Editor in Chief
Prof. Dr. Asli UÇAR (PhD)
Department of Nutrition and Dietetics, Faculty of Health Sciences, University of Ankara, Ankara, Turkey

Bibliography

Prof. Dr. Asli UÇAR was awarded the PhD degree in Nutrition Sciences in 2006. She has excellent knowledge of food infection, hygiene of catering, attitudes of women towards food safety and Turkish perception of organic foods. Her major research area is food safety, nutrition science, food questionnaire and food hygiene. Prof. Asli UÇAR has recently completed three projects entitled Investigation of Effect of Some of Group B Vitamins and Vitamin D on Depression Level, Evaluation of Nutritional Status and Eating Habits of Children with Autism and Investigation of Effect Exposure to Steviol Glycoside on Oxidative Damage Index Parameters, Paraxonase Enzyme and Some Genetic Parameters in Balb/c Mice. Moreover, she partially contributed to compile the book (ISBN 978-953-51-2277-7) entitled Food Safety, Problems and Solutions - Significance, Prevention and Control of Food Related Diseases and wrote book chapter (ISBN 978-953-307-764-2) entitled Organic Food and Agriculture - New Trends and Developments in the Social Sciences. 

Journal Highlights
Key title: Advanced Food and Nutrition Sciences 
Abbreviation: Adv Food & Nutr Sci
Frequency: Annual

Current Volume: 2 (2017)
Next volume: 3 (2018)
Back volumes: 1-2
Starting year: 2016
Nature: Online
Submission: Online
Language: English

Indexing

Subject & Scope

  • Advanced Food Processing Technologies
  • Amino acid Supplements
  • Animal & Plant Nutrition
  • Bodybuilding Nutrition
  • Celery Nutrition
  • Childhood Obesity
  • Clinical Sports Nutrition
  • Creatine Sports Nutrition
  • Dates Nutrition
  • Diabetes Nutrition
  • Dietary Intake
  • Eating Disorders
  • Fatigue Disorders
  • Fermentation Technology
  • Fitness Nutrition
  • Food Addiction
  • Food Allergy Studies
  • Food Biotechnology Studies
  • Food Biotechnology and Nutrition
  • Food Chemical Engineering
  • Food Fortification
  • Food Intolerance
  • Food Nanotechnology
  • Food Process Engineering
  • Food Processing
  • Food Production
  • Food Safety regulations
  • Food Science
  • Food Security/ Safety
  • Food Technology
  • Food Toxicology
  • Gym Suppliments
  • Health Nutrition
  • Herbal Supplements
  • Human Nutrition
  • Iodine Deficiency Disorders
  • Malnourishment
  • Malnutrition
  • Mass Spectrometry in Food Technology
  • Molecular Nutrition
  • Natural Suppliments
  • Nutrient Deficiencies
  • Nutrigenomics
  • Nutrition Cancer
  • Nutrition Economics
  • Nutrition Sport Fitness
  • Nutrition and Diabetes
  • Nutrition and Food Sciences
  • Nutrition in Developing Countries
  • Nutritional Epidemiology
  • Nutritional Values
  • Nutritionist Communications
  • Oatmeal Nutrition
  • Obesity Prevention
  • Obesity and Weightloss
  • Potato Nutrition
  • Protein Diet
  • Protein Suppliments
  • Public Health Nutrition
  • Sonic Nutrition
  • Sports Nutrition
  • Sports Nutrition Suppliments
  • Vitamin B12 Deficiency
  • Vitamin Supplement

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