PROCESS STRATEGIES FOR ALKALINE LIPASE PRODUCTION USING Aspergillus Niger MTCC 2594
Suraj Abraham ,2,3*; Numbi Ramudu Kamini3 and Marichetti Kuppuswami Gowthaman3
1. Biological Sciences Group, Faculty division III, Birla Institute of Technology & Science, Vidhya Vihar Campus, Pilani, 333 031, India
2. Department of Anatomy & Cell biology, College of Medicine, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada
3. Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, 600 020, India
Keywords: Aspergillus niger MTCC 2594, substrate inhibition, Fed batch, Production, Shake flask, Submerged stir tank fermenter]
We report scale-up production of alkaline lipases from Aspergillus niger MTCC 2594 using submerged stir tank fermenter (SSTF). The batch culture experiments and biomass studies suggest that olive oil used in the process reduced enzyme yield. We found that the addition of olive oil intermittently might be a potential fed-batch strategy to improve lipase production using Aspergillus niger MTCC 2594.