IN VIVO ANTIOXIDANT ACTIVITY OF PHYLLANTHUS EMBLICUS AGAINST CISPLATIN INDUCED OXIDATIVE STRESS IN MICE
Mamuna Naz, Uzma Saleem, Bashir Ahmad
Faculty of Pharmacy, The University of Lahore, Lahore-Pakistan.Assistant Professor, Faculty of Pharmaceutical Sciences, G. C. University, Faisalabad-Pakistan. Director / Professor, Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore-Pakistan.
Keywords: In vivo antioxidant activity, hypolipidemic, hepato-renal curative
Background: Plants are rich source of antioxidants. They can ameliorate the oxidative stress induced complications. Cisplatin is a cytotoxic drug which produced unwanted effects in the patients due to generation of free radicals inside the body. Phyllanthus emblicuspossessed in vitro antioxidant activity.
Objective: The current study was aimed to explore in vivo antioxidant potential of Phyllanthus emblicus against the oxidative stress induced by cisplatin in mice.
Method: oxidative stress was induced in micewith acute toxic dose (10 mg/kg) of cisplatin given i.p. Animals were divided into five groups (n = 5). Group I: negative control, group II: positive control group III and IV were given methanol extract ofPhyllanthus emblicus (250- & 500 mg/kg; orally respectively) and group V was standard group receiving orally vitamin C & E (200 mg/kg each) for 20 days. On 21st day, animals were sacrificed and oxidative stress biomarkers were quantified.
Result: Phyllanthus emblicusextract showed vigorous in vivo antioxidant effect at 500mg/kg by increasing the SOD, CAT, and GSH (antioxidant enzymes) levels in heart, liver, kidney and brain homogentaes and MDA level decreased. Plant also displayed a cure against oxidative stress induced changes in renal, liver and lipid profile parameters.
Conclusion: Phyllanthus emblicusraised antioxidant enzyme levels in mice. It manifested hypolipidemic, hepato-renal curative effects. Its adjuvant use with standard therapies may help to resolve unwanted effects.
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