Minky Mukhija, Ajudhia Nath Kalia
ISF College of Pharmacy, Ferozepur Road, Moga, Punjab, India. Punjab Technical University, Kapurthala, Punjab, India
Keywords: Antioxidant activity, DPPH assay, NO assay, Zanthoxylum alatum,

In recent years much attention has been devoted to natural antioxidant and their association with health benefits. Plants are the potential source of natural antioxidants. The aim of this study was to assess the antioxidant activity (in vitro) of four extracts from stem bark of Zanthoxylum alatum Roxb. and to determine phenolic content of all extracts. The best antioxidant potential and phenolic content were found in Petroleum ether and ethyl acetate extract. Comparative antioxidant potential was evaluated using DPPH, Nitric Oxide scavenging assay and ferric reducing power assay. Estimation of the total phenolic content was performed using Folin-Ciocalteu’s method. Petroleum ether and ethyl acetate extract gave an IC50 of 85.16±1.05, 72.39±1.53 and 99.25±2.53, 94.81±2.56 for DPPH and Nitric oxide scavenging activities respectively. The petroleum ether and ethyl acetate extracts showed good reducing power with increasing concentration. Both extracts also gave the maximum percentage of phenolic content (equivalent to that of gallic acid) when compared to other extracts. All the antioxidant assays and phenolic content estimation studies revealed that petroleum ether and ethyl acetate extracts were the most promising and significant extract among petroleum ether, chloroform, ethyl acetate and methanol extracts.

Article Information

Identifiers and Pagination:
First Page:357
Last Page:365
Publisher Id:19204159.6:4.2014
Article History:
Received:June 19, 2014
Accepted:August 02, 2014
Collection year:2014
First Published:October 1, 2014

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Editor in Chief
Prof. Dr. Cornelia M. Keck (Philipps-Universität Marburg)
Marburg, Germany


Welcome to the research group of Prof. Dr. Cornelia M. Keck in Marburg. Cornelia M. Keck is a pharmacist and obtained her PhD in 2006 from the Freie Universität (FU) in Berlin. In 2009 she was appointed as Adjunct Professor for Pharmaceutical and Nutritional Nanotechnology at the University Putra Malaysia (UPM) and in 2011 she obtained her Venia legendi (Habilitation) at the Freie Universität Berlin and was appointed as a Professor for Pharmacology and Pharmaceutics at the University of Applied Sciences Kaiserslautern. Since 2016 she is Professor of Pharmaceutics and Biopharmaceutics at the Philipps-Universität Marburg. Her field of research is the development and characterization of innovative nanocarriers for improved delivery of poorly soluble actives for healthcare and cosmetics. Prof. Keck is executive board member of the German Association of Nanotechnology (Deutscher Verband Nanotechnologie), Vize-chairman of the unit „Dermocosmetics“ at the German Society of Dermopharmacy, active member in many pharmaceutical societies and member of the BfR Committee for Cosmetics at the Federal Institute for Risk Assessment (BfR).

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Abbreviation: J App Pharm
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