Rifat-uz-Zaman, Maida Ghaffar, Tehrim Fayyaz, Shumaila Mehdi
Faculty of Pharmacy and Alternative Medicine, the Islamia University of Bahawalpur-Pakistan
Keywords: Antioxidant activity, DPPH, Folin-Ciocalteu, medicinal plants, W. somnifera, E. prostrate and G. herbasceum.

Traditional medicinal plant, Withania somnifera, Eclipta prostrata and Gossypium herbasceum were analyzed for reducing power ability as an antioxidant using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and for total phenolics using the Folin-Ciocalteu method. The results of the analysis show that W. somnifera, E. prostrate and G. herbascium have percentage antioxidant activity (AA%) of 98.12±0.50, 96.45±0.45 and 90.62±0.35 respectively. W. somniferam and E. prostrate show higher while Gossypium herbasceum less activity than those of Gallic (92.92±0.55) and Ascorbic acids (94.81±0.56) used as standards. W. somnifera, E. prostrata and G. herbascium also show high reductive potential of 0.99, 0.94 and 0.90nm at the same concentration with Gallic acid whose reductive potential was 0.93nm. The total phenolic determination shows that W. somnifera, E. prostrate and G. herbascium have the phenolic content of 14, 12 and 11mg/g GAE respectively. The results of this analysis reveal the fact that plants are rich sources of natural antioxidant.

Article Information

Identifiers and Pagination:
First Page:9
Last Page:19
Publisher Id:JAppPharm (2011 ). 3. 9-19
Article History:
Received:September 3, 2010
Accepted:November 27, 201
Collection year:2010
First Published:January 11, 2011

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Editor in Chief
Prof. Dr. Cornelia M. Keck (Philipps-Universität Marburg)
Marburg, Germany


Welcome to the research group of Prof. Dr. Cornelia M. Keck in Marburg. Cornelia M. Keck is a pharmacist and obtained her PhD in 2006 from the Freie Universität (FU) in Berlin. In 2009 she was appointed as Adjunct Professor for Pharmaceutical and Nutritional Nanotechnology at the University Putra Malaysia (UPM) and in 2011 she obtained her Venia legendi (Habilitation) at the Freie Universität Berlin and was appointed as a Professor for Pharmacology and Pharmaceutics at the University of Applied Sciences Kaiserslautern. Since 2016 she is Professor of Pharmaceutics and Biopharmaceutics at the Philipps-Universität Marburg. Her field of research is the development and characterization of innovative nanocarriers for improved delivery of poorly soluble actives for healthcare and cosmetics. Prof. Keck is executive board member of the German Association of Nanotechnology (Deutscher Verband Nanotechnologie), Vize-chairman of the unit „Dermocosmetics“ at the German Society of Dermopharmacy, active member in many pharmaceutical societies and member of the BfR Committee for Cosmetics at the Federal Institute for Risk Assessment (BfR).

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Abbreviation: J App Pharm
Frequency: Annual 
Current Volume: 9 (2017)
Next scheduled volume: December, 2018 (Volume 10)
Back volumes: 1-9
Starting year: 2009
Nature: Online 
Submission: Online  
Language: English

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