Md. Rezanur Rahman, Tania Islam, Hossain Md Faruquee*, Sharmin Akhter, and Md Anwarul Haque
1 Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh. 2 Department of Applied Nutrition and Food Technology, Faculty of Applied Science and Technology, Islamic University, Kushtia-7003, Bangladesh.
Keywords: Alzheimer’s disease; Glycomis pentaphylla; anticholinesterase; anti-oxidant; antibacterial; radical scavenging; lipid peroxidation.

There is a tremendous unmet need to discover more potent and safe drugs for the treatment of Alzheimer’s disease (AD). Reduced cholinergic activity and oxidative stress have been recognized as a major contributing factor in the pathogenesis of AD. Therefore, inhibition of cholinesterase and oxidation are the two promising strategies in the development of a drug for AD. This study determined the anti-acetylcholinesterase (AChE) activity, anti-butyrylcholinesterase (BChE) activity, DPPH free radical scavenging and antioxidant properties of Glycomis pentaphylla (Rutaceae). The objective of this study is to measure G. pentaphylla anti-AChE, anti-BChE, DPPH free radical scavenging, lipid peroxidation inhibition, antibacterial potentials to find out the MIC (minimum inhibitory concentration) against different pathogenic bacteria. G. pentaphylla leaf extract (GPEx) is exploited in the presented research to estimate its anticholinesterase, antioxidant potentials, and antibacterial properties. The cholinesterase inhibitory properties was quantified by modified Ellman method, and antioxidant potentials were evaluated by the assay of radical scavenging, and inhibition of lipid peroxidation. The antibacterial activity and minimum inhibitory concentration (MIC) were determined using agar well diffusion method. The methanolic extract exhibited significant dual acetylcholinesterase (AChE) and butyryl cholinesterase (BChE) effect. The IC50 values of AChE and BChE were 325.1±0.91, and 42.14±3.31 µg/ml. Furthermore, the extract showed radical scavenging ability, and lipid peroxidation inhibitory effect. The IC50 values of the extract for DPPH and hydroxyl free radical scavenging, and lipid peroxidation inhibition assay were 95.6±0.68, 198.0±1.39, and 288.7±0.91 µg/ml, respectively. Phytochemical screening of the extract revealed the presence of significant total phenolics and flavonoids contents. Additionally, the extract showed good effect with the zone of inhibition ranging 12–16 mm in diameter against Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus. The tested sample reflects potential antioxidative and anticholinesterase inhibitory potentiality which may warrant its effectiveness in the treatment of AD along with good antibacterial properties.

Article Information

Identifiers and Pagination:
First Page:17
Last Page:30
Publisher Id:JAppPharm (2017 ). 9. 17-30
Article History:
Received:October 9, 2017
Accepted:December 3, 2017
Collection year:2017
First Published:December 17, 2017

© 2016 The Author(s). This open access article is distributed under a Creative Commons Attribution (CC-BY) 4.0 license. You are free to: Share — copy and redistribute the material in any medium or format Adapt — remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. No additional restrictions You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits
Editor in Chief
Prof. Dr. Cornelia M. Keck (Philipps-Universität Marburg)
Marburg, Germany


Welcome to the research group of Prof. Dr. Cornelia M. Keck in Marburg. Cornelia M. Keck is a pharmacist and obtained her PhD in 2006 from the Freie Universität (FU) in Berlin. In 2009 she was appointed as Adjunct Professor for Pharmaceutical and Nutritional Nanotechnology at the University Putra Malaysia (UPM) and in 2011 she obtained her Venia legendi (Habilitation) at the Freie Universität Berlin and was appointed as a Professor for Pharmacology and Pharmaceutics at the University of Applied Sciences Kaiserslautern. Since 2016 she is Professor of Pharmaceutics and Biopharmaceutics at the Philipps-Universität Marburg. Her field of research is the development and characterization of innovative nanocarriers for improved delivery of poorly soluble actives for healthcare and cosmetics. Prof. Keck is executive board member of the German Association of Nanotechnology (Deutscher Verband Nanotechnologie), Vize-chairman of the unit „Dermocosmetics“ at the German Society of Dermopharmacy, active member in many pharmaceutical societies and member of the BfR Committee for Cosmetics at the Federal Institute for Risk Assessment (BfR).

Journal Highlights
Abbreviation: J App Pharm
doi: http://dx.doi.org/10.21065/19204159
Frequency: Annual 
Current Volume: 9 (2017)
Next scheduled volume: December, 2018 (Volume 10)
Back volumes: 1-9
Starting year: 2009
Nature: Online 
Submission: Online  
Language: English

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