Afendi Dahlan, Mohd Asyraf Mohd Razali
Department of Pharmaceutics, Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, Lot 3410 Jalan Teknokrat 3, Cyberjaya 63000, Selangor, Malaysia
Keywords: Antibacterial Activity, Pomegranate (Punica Granatum)

Purpose. Previous studies have shown that Punica granatum (P. granatum) extract exhibited antimicrobial activity against a wide range of pathogenic microorganisms. Thus, the aim of study was to formulate and determine the effectiveness of antimicrobial properties of gel containing P. granatum methanol extract against selected common skin pathogens including Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans. Methods. Extraction process utilizes P. granatum peel and methanol as solvent. Using the disc diffusion method, different concentrations (25 %w/v, 50 %w/v, 75 %w/v and 100 %w/v) of P. granatum extract were initially tested against S. aureus, S. epidermidis, P. aeruginosa and C. albicans for their antibacterial activity. Gel formulations were then prepared with varying concentration of the extract as active ingredient. Antimicrobial activity of P. granatum gel was determined by using agar well diffusion method. The gel formulations were stored at 4°C, 25°C and 37°C for one month. Evaluation on the pH, physical characteristic and antimicrobial activity were then conducted on the gel formulations. Results. Both extract and gel showed highest antibacterial activity against S. epidermidis followed by S. aureus and then P. aeruginosa. Surprisingly no antifungal activity was observed against C. albicans. The concentration of extract alone and in gel were directly proportional to antibacterial activity. All gels formulated showed satisfactory physical characteristics even after a month of storage at different temperature. As expected, gel stored at 4°C showed the least decrease in antibacterial activity. Conclusions. P. granatum has potential to be formulated as gel to treat bacterial skin infection caused by S. aureus, S. epidermidis and P. aeruginosa. Further studies are required in order to optimize the antimicrobial activity of P. granatum gel.

Article Information

Identifiers and Pagination:
First Page:13
Last Page:23
Publisher Id:19204159.7:1.2015
Article History:
Received:October 21,2014
Accepted:November 11,2014
Collection year:2015
First Published:January 1, 2015

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Editor in Chief
Prof. Dr. Cornelia M. Keck (Philipps-Universität Marburg)
Marburg, Germany


Welcome to the research group of Prof. Dr. Cornelia M. Keck in Marburg. Cornelia M. Keck is a pharmacist and obtained her PhD in 2006 from the Freie Universität (FU) in Berlin. In 2009 she was appointed as Adjunct Professor for Pharmaceutical and Nutritional Nanotechnology at the University Putra Malaysia (UPM) and in 2011 she obtained her Venia legendi (Habilitation) at the Freie Universität Berlin and was appointed as a Professor for Pharmacology and Pharmaceutics at the University of Applied Sciences Kaiserslautern. Since 2016 she is Professor of Pharmaceutics and Biopharmaceutics at the Philipps-Universität Marburg. Her field of research is the development and characterization of innovative nanocarriers for improved delivery of poorly soluble actives for healthcare and cosmetics. Prof. Keck is executive board member of the German Association of Nanotechnology (Deutscher Verband Nanotechnologie), Vize-chairman of the unit „Dermocosmetics“ at the German Society of Dermopharmacy, active member in many pharmaceutical societies and member of the BfR Committee for Cosmetics at the Federal Institute for Risk Assessment (BfR).

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Abbreviation: J App Pharm
Frequency: Annual 
Current Volume: 9 (2017)
Next scheduled volume: December, 2018 (Volume 10)
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Starting year: 2009
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