BIOSYNTHESIS OF Zn BACITRACIN BY Bacillus licheniformis UNDER SUBMERGED FERMENTATION USING WHEAT BRAN
Arifa Tahir, Hafiza Hifsa Roohiand Tahira Aziz Mughal
1 Environmental Science Department, Lahore College for Women University, Lahore, Pakistan. 2 Botany Department, Lahore College for Women University, Lahore, Pakistan.
Keywords: Zn bacitracin, Biosynthesis, Submerged fermentation, Optimization, Purification
Abstract

Bacitracin is a potent antibiotic which is used in combination with other antimicrobial agents. Bacitracin in the form of its zinc salt is used as feed additive, growth promoter and for reduction of diseases in poultry. In the present study, twenty five bacterial strains capable of producing bacitracin were isolated from milk samples by heat shock method. Among all bacterial strains, the isolate no. 21 was found to be the most potential strain; it was identified and designated as Bacillus licheniformisBCL-21. Different fermentation media were evaluated for antibiotic production under submerged fermentation in shake flasks. The medium M1 gave higher antibiotic production (245.5 IU/ml) than M2(186.0 IU/ml) and M3(202.2 IU/ml). Different parameters were optimized in shake flask studies. The antibiotic production was 271.2±1.51 IU/ml by using 24 h old inoculum. Inoculum size was optimized to be 6.0 % ((274.0±1.89 IU/ml). The optimum pH was found to be 8.0 and bacitracin production was 245.5±0.58 IU/ml. At 37oCtemperature, production was295.0±1.34 IU/ml. Antibiotic activity was observed to be 283.9±1.43 IU/mlafter 48 h of incubation. The optimum agitation speed and working volume were observed to be 150 rpm(207.0±0.85 IU/ml) and 100 mL(232±1.29 IU/ml) respectively. Partial purification of bacitracin from fermentation broth was successfully done by precipitation method.

Article Information

Identifiers and Pagination:
Year:2012
Volume:4
First Page:26
Last Page:37
Publisher Id:JAppPharm (2012 ). 4. 26-37
Article History:
Received:September 17, 2011
Accepted:December 15, 2011
Collection year:2011
First Published:January 10, 2012


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Editor in Chief
Prof. Dr. Cornelia M. Keck (Philipps-Universität Marburg)
Marburg, Germany

Bibliography

Welcome to the research group of Prof. Dr. Cornelia M. Keck in Marburg. Cornelia M. Keck is a pharmacist and obtained her PhD in 2006 from the Freie Universität (FU) in Berlin. In 2009 she was appointed as Adjunct Professor for Pharmaceutical and Nutritional Nanotechnology at the University Putra Malaysia (UPM) and in 2011 she obtained her Venia legendi (Habilitation) at the Freie Universität Berlin and was appointed as a Professor for Pharmacology and Pharmaceutics at the University of Applied Sciences Kaiserslautern. Since 2016 she is Professor of Pharmaceutics and Biopharmaceutics at the Philipps-Universität Marburg. Her field of research is the development and characterization of innovative nanocarriers for improved delivery of poorly soluble actives for healthcare and cosmetics. Prof. Keck is executive board member of the German Association of Nanotechnology (Deutscher Verband Nanotechnologie), Vize-chairman of the unit „Dermocosmetics“ at the German Society of Dermopharmacy, active member in many pharmaceutical societies and member of the BfR Committee for Cosmetics at the Federal Institute for Risk Assessment (BfR).

Journal Highlights
Abbreviation: J App Pharm
doi: http://dx.doi.org/10.21065/19204159
Frequency: Annual 
Current Volume: 9 (2017)
Next scheduled volume: December, 2018 (Volume 10)
Back volumes: 1-9
Starting year: 2009
Nature: Online 
Submission: Online  
Language: English

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