EFFECT OF DICLOFENAC SODIUM ANGIOGENESIS USING CHORIOALLANTOIC
MEMBRANE (CAM) ASSAY
Iradat Hussain 1,2, Muhammad Ovais Omer2, Muhammad Ashraf2, Habib-Ur-Rehman2
1 Department of Pharmacy, Margalla Institute of Health Sciences, Islamabad, Pakistan
2 Faculty of Biosciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
Keywords: Diclofenac sodium, Angiogenesis, Chorio-allantoic Membrane (CAM) assay.
Angiogenesis, the formation of new blood vessels, is a hallmark of almost all neoplastic
and non-neoplastic degenerative diseases. This process supports normal physiology as
well as it contributes in progression of different diseases. Angiogenesis contributes in
growth of tumor and progressive arthritis. Inflammatory mediators are involved in
cancer induced angiogenic process. Cyclo-oxygenases promote these mediators which
help in cell migration and endothelial cell spreading. To explore the role of diclofenac
sodium in angiogenesis we have used in vitro Chorioallantoic membrane assay. A novel
image probing system SPIP (scanning probe image processor) was utilized for
assessment and quantification of structural changes in CAMs. Fourteen parameters of
3D surface roughness were also evaluated for quantification. Application of diclofenac
sodium on Chorioallantoic membrane at day six of incubation (0.7% concentration of
diclofenac sodium) showed anti-angiogenic effect. Results showed marked changes in
architecture of CAMs, thinning of primary, secondary and tertiary blood vessels,
reduction in surface roughness parameters, increase in kurtosis of surface, and decrease
in Abbott curve. The substantial quantities of diclofenac sodium use locally may exhibit
anti-angiogenic activity in the same manner those seen in in-vitro and explain its
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