ANTIBACTERIAL AND SYNERGISTIC STUDIES OF SALSOLA KALI
Tahira Mughal1, Ismat Naeem2, Muhammad Tahir Aziz3, Afshan Ahsan1
1. Department of Botany, Lahore College for Women University, Jail Road, Lahore, Pakistan 2. Department of Chemistry, Lahore College for Women University, Jail Road, Lahore, Pakistan 3. Shaukat Khanum Memorial Cancer Hospital and Research Center, Lahore Pakistan
Keywords: Salsola kali, Antibacterial activity, Synergistic activity Heliotropium strigosum, Galium asperuloides, Senecio chrysanthemoides,
Abstract

Salsola kali is an annual herb, found near the Bahawalpur (Cholistan desert) South Punjab, Pakistan. Extract of aerial part of the plant in methanol was tested for antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae, Bacillus subtilis, Streptococcus mutans and Sarcina lutae. Methanol was found to be best antimicrobial solvent. Minimum Inhibitory Concentration (MIC) of methanolic extract was determined and found to be bactericidal in concentration of 0.5 µg/ml against Staphylococcus aureus, S. pneumoniae, Bacillus subtilis and Streptococcus mutans. Synergistic antibacterial activity of methanolic extracts was tested with respective solvent extracts of aerial parts of the Heliotropium strigosum, Galium asperuloides and Senecio chrysanthemoides synergistically showed best antibacterial activity against all the bacteria (0.5 µg/ml). The extract of Salsola kali and the methanolic extract of Galium asperuloides showed best activity against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Streptococcus mutans (0.5µg/ml). Synergistically Salsola kali with Senecio chrysanthemoides showed best activity against all the bacterial strains (0.5µg/ml) except S. lutae and S. mutans . Salsola kali with Heliotropium strigosum showed best activity against E. coli, S. pneumoniae, B. subtilis, S. lutae and S. mutans (0.5µg/ml) and inhibited the growth of Staphylococcus aureus and Pseudomonas aeruginosa.

Article Information

Identifiers and Pagination:
Year:2010
Volume:2
First Page:18
Last Page:26
Publisher Id:JAppPharm (2010 ). 2. 18-26
Article History:
Received:July 3, 2009
Accepted:November 27, 2009
Collection year:2009
First Published:January 13, 2010

INTRODUCTION
According to World Health Organization, (Santose, et. al., 1995) medicinal plants would be the best source
to obtain a variety of drugs. About 80% of individuals from developed countries use traditional medicines,
which have compounds derived from medicinal plants. Therefore, such plants should be investigated to
better understand their properties, safety and efficiency (Elof, et al., 1998).
Klein (1998) has taken view and supported it by strong experimental evidence, that synergism occurs only
when the microorganism tend to become drug-fast and is related to inhibition by the second of resistance
microorganism surviving the action of first. Secondly, when drug-fastness does not develop with some
facility, synergism commonly does not occur and some degree of antagonism may be observed. The
consequence of combining drug is dependent upon the strains of micro-organisms upon the condition under
which the organism is subjected to the action of drug.
Salsola kali (Family: Chenopodiaceae) is an annual herb commonly found in Bahawalpur (Cholistan
desert) South Punjab, Pakistan. This plant has medicinal importance and is used in traditional medicinal
system of their native region by various hakims to fight against disease. This plant is used to investigate the
antibacterial activity because of its medicinal properties.
Nascimento, G.F, et al., (2000) evaluated the antimicrobial activity of plant extracts and phytochemicals
with antibiotic susceptible and resistant microorganisms. The possible synergistic effects when associated
with antibiotics were studied with Achillea millifolium (yarrow), Melissa officinalis (lemon-balm), Ocium
basilucum (basil), Psidium guajava (guava), Punica granatum (pomegranate), Rosmarinus officinalis
(rosemary), Salvia officinalis (sage), Syzgyum joabolanum (jambolan) and Thymus vulgaris (thyme). The
highest antimicrobial potentials were observed for the extracts of Caryophyllus aromaticus and Syzygyum
joabolanum, which inhibited 64.2 and 57.1% of the tested microorganisms, respectively, with higher
activity against antibiotic resistance bacteria (83.3%).Association of antibiotics and plant extracts showed
synergistic antibacterial activity against antibiotic resistance bacteria. Pseudomonas aeruginosa was
inhibited by clove, jambolan, pomegranate and thyme extracts. This inhibition was observed with the
individual extracts and when they were used in lower concentrations with ineffective antibiotics.
Soberon J.R. et al., (2007) determined the antibacterial and cytotoxic activities of aqueous and ethanolic
extracts of northwestern Argentinian plants used in folk medicine and compared with different commercial
antibiotics. Tripodanthus acutifolius aqueous extract has lower inhibitory concentrations than cefotaxim
against Acinetobacterfreundii. Tripodanthus acutifolius tincture showed lower MIC and minimal
bactericidal concentration (MBC) than cefotaxim for Pseudomonas aeruginosa. This extract also showed a
MIC/MBC lower than oxacillin for Staphylococcus aureus. The cyto-toxicity of all extracts was compared
with that of commercial antibiotics. Rutin (3,3',4',5,7-pentahydroxy flavone 3-beta-rhamnosilglucoside ,isoquercitrin
(3,3',4',5,7-pentahydroxyflavone 3-beta-glucoside) and a terpene would be partially responsiblefor the antibacterial activity of T. acutifolius infusion.
Bonjar, et al., (2004) reported that forty-five species of 29 plant families used in the traditional medicine
by Iranian people, showed antibacterial activities against one or more of the bacterial species: Bacillus
cereus, Bacillus pumilus, Bordetella bronchiseptica, Escherichia coli, Klebsiella pneumoniae, Micrococcus
luteus, Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens, Staphylococcus aureus
and Staphylococcus epidermidis. No plant showed activity against Serratia marcescens, Bordetella
bronchiseptica being the most susceptible species. All extracts showed the same activity 18 months later.
Camporese, et al., (2003) reported that twenty-one extracts from seven herbal drugs, Aristolochia trilobata
(Aristolochiaceae) leaves and bark, Bursera simaruba (Burseraceae) bark, Guazuma ulmifolia
(Sterculiaceae) bark, Hamelia patens (Rubiaceae) leaves and Syngonium podophyllum (Araceae) leaves and
bark, used in traditional medicine of Belize (Central America) as deep and superficial wound healers, were
evaluated for their anti-bacterial properties. Activity was tested against standard strains of Escherichia coli
ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and
Enterococcus faecalis ATCC 29212. Almost all the extracts were able to inhibit the growth of one or more
of the bacterial strains, except that of Enterococcus faecalis.


MATERIAL AND METHODS
Microbial strains of Staphylococcus aureus (ATCC 25923) (S.aureus). Escherichia coli (ATCC 2592) (E.
coli), pseudomonas aeruginosa (ATCC 27853) (P. aeruginosa), Streptococcus pneumoniae (ATCC 49619)
(S. pneumoniae) Bacillus subtilus (ATCC 6051) (B. subtilus) and Sarcina lutae ATCC 9341 (S. lutae) and
Streptococcus mutans (ATCC 020572) were obtained from Pediatrics, Microbiology Laboratory, Mayo
hospital Lahore.
Plant material
Fresh plants of Salsola kali (whole plant (PM # 086) was collected from South Punjab (Bahawalpur Road )
on 6th June 2005 and Senecio chrysanthemoides (whole plant, PM # 185) and Galium asperuloides (PM #
0234) were collected from area between Nathia Gali and Khanuspure , Muree Hill , Pakistan on 2nd
October 2005. The plants were identified by Mir Ajab Ali Khan, Professor of Botany Quaid-e-Azam
University, Islamabad, Pakistan, Dr. Zaheer-ud-din , Professor of Botany Government College Lahore,
Pakistan and voucher specimen were deposit in Prem Madan Herbarium of Lahore College for Women
University, Lahore Pakistan.
Salsola kali (whole Plant), Senecio chrysanthemoides (whole Plant) , Galium asperuloides (whole plant)
and Heliotropium strigosum (whole Plant) were air dried, finely gained extracted methanol by soxhelt
extraction to yield 15% ,20%, 18% and 25% solvent free extract.
Anti bacterial activity was determined by agar well diffusion method [Norsel and Messley .1977]. This test
was performed in triplicate by spreading 12-18 hour old pathogenic bacterial cutters containing
approximately 106 - 1010 colony forming unit (CFU/ml) on the surface of nutrient agar plates well (4mm)
were dug in the media with the help of sterile metallic borer.
Test samples of different concentrations prepared in Methanol were added (50µl) in their respective wells
pure methanol was used as negative control (3mm) other wells were supplemented with reference
compounds i.e. Ampicillin, Amoxicillin, Levofloxin, Tetracycline, Vancomycin, Ciprofloxacin and
Penicillin as positive control.
Synergistic Activity against bacterial strains was determined by taking equal amount (50µl (1:1))of plant
extracts by agar well diffusion method as described before.


RESULTS AND DISCUSSIONS
Seven pathogenic bacterial strains (Staphylococcus aureus, Escherichia. coli, Bacillus subtilis,
Streptococcus pneumoniae , Sarcina lutae, Streptoccus mutans and Pseudomonas aeruginosa) were used
in this study and methanolic extract of Salsola kali with three combinations of methanolic extracts of
other plants (Salsola kali + Galium asperuloides, Salsola kali + Senecio chrysanthemoides and Salsola kali
+ Heliotropium strigosum with concentrations (250, 100, 50, 10, 5, 1.0, 0.5 µg/ml) were used against each
of the seven bacterial strains.
The crude methanolic extract of Salsola kali showed highest activity against S.mutans, S.aureus, B.subtilis
and S. pneumoniae while it showed moderate bactericidal activity against P.aeruginosa. The crude
methanolic extract of Salsola kali inhibits the growth of S.lutae and E. coli. (Table-01&05)
Table – 01 Zone of inhibitions of crude methanol extracts of Salsola kali
The crude methanolic extract of Senecio chrysanthemoides showed highest antibacterial activity against
S.aureus, S.mutans and S.pneumoniae . The plant extract inhibits the growth of P.aeruginosa, E.coli
,B.subtilus and S.lutae (Table -02&05)
Table -02 Zone of inhibition of crude methanol extracts of Senecio chrysanthemoides
The methanolic extract of Galium asperuliodes showed moderate activity against S.aureus, S. pneumoniae,
S.lutae and E.coli . While this extract showed good activity by increase the concentration of the extract
with P.aeruginosa , S.mutans and B.subtilis. (Table-04 &05)
Table -04 Zone of inhibition of crude methanol extracts of Galium asperuliodes
Synergistic activity: The crude methanolic extract of Salsola kali and Heliotropium strigosum showed
good activity against S.lutae, S.pnumiae, E.coli , B.subtilus and S.mutans (0.5µg/ml). The crude
methanolic extract of Salsola kali with Heliotropium strigosum showed moderate activity against S.aureus
and P.aeruginosa . (Table-06& 09)
Table -06 Zone of inhibition of crude methanol extracts of Salsola kali and Heliotropium strigosum
The crude methanolic extract of Salsola kali and Senecio chrysanthemoides showed highest activity
against E.coli, P.aeruginosa B.subtilus, S.aureus and S.pneumoniae but inhibit the growth of S.lutae and
S.mutans (Table- 07 & 09)
The methanolic extract of Salsola kali and Galium asperuloides showed highest activity against
P.aeruginosa E.coli, Sarcina lutae, Streptococcus mutans, Streptococcus pneumoniae and B.subtilis . The
Plant MIC value ranges from 0.5µg/µl-1.0µµg/l. While this extract showed moderate activity against
S.aerus .( Table 08 & 09)
Table -08 MIC value of crude methanol extracts of Salsola kali and Galium asperuloides.
The MIC values of test medicinal plants showed best activity as compared with the different discs of the
antibiotics (Ampicillin, Amoxicillin, Levofloxacin, Tetracycline, Vancomycin, Ciprofloxacin and
Penicillin) against the test strains of bacteria. (Table -10)


REFERENCE
1. Naeem I, T. Mughal, U.Maslahuddin , B. Mateen , and H.Ikram . Nov.2-3, 2007. Synergistic
Activity of Withania coagulans with some other Medicinal Plants of Pakistan, International
Conference of Chemistry, Lahore College for Women University. Abstract: page 33
2. Siddique Z, A. Sauteau , C.Hellio and I.Naeem . 2008. Antibacterial activity of Hypericum
perforatum, Proceedings of International Seminar on Medicinal Plants, Lahore College for Women
University). Abstract: page 5.
3. Camporese A. Balick M J., R. Arvigo, R. G. Esposito, N. Morsellino, F. De Simone and A. Tubaro,
2003, Screening of anti-bacterial activity of medicinal plants from Belize (Central America) Journal
of Ethnopharmacology, 87 (1) 103-107
4. Bonjar. G.,H. Shahidi, 2004 Evaluation of Antibacterial Properties of Iranian Medicinal-Plants
against Micrococcusluteus, Serratia marcescens, Klebsiella pneumoniae and Bordetella
bronchoseptica Asian Journal of Plant Sciences 3 (1): 82-86ISSN 1682-3974
5. Ellof, J.N.1998 which extractant should be used for the screening and isolation of antimicrobial
components from plants? J. Ethnopharmacol. 60, 1-6, 1998
6. Klein, J.P.; Scholler, M. 1998. “Recent Advances in the Development of a Streptococcus mutans
Vaccine”. European Journal of Epidemiology 4 (4): 419–425. doi:10.1007/BF00146392. Retrieved
on 2007-05-15.
7. Nascimento, S.C.; Chiappeta, A.; Lima, R.M.O.C. 2000 Antimicrobial and cytotoxic activities in
plants from Pernambuco, Brazil. Fitoterapia 61, 353-355.
8. Santos, P.R.V.; Oliveira, A.C.X.; Tomassini, T.C.B. 1995 Controle microbiógico de produtos
fitoterápicos. Rev. Farm. Bioquím. 31, 35-38.
9. Soberon J.R, Sampietro D.A, Quiroqa E.N, Vattuone M.A, 2007, Antibacterial activity of plant
extracts from northwestern Argentina, Journal ofApplied Microbiology. vol;102(6),1450-61


© 2016 The Author(s). This open access article is distributed under a Creative Commons Attribution (CC-BY) 4.0 license. You are free to: Share — copy and redistribute the material in any medium or format Adapt — remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. No additional restrictions You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits
Editor in Chief
Prof. Dr. Cornelia M. Keck (Philipps-Universität Marburg)
Marburg, Germany

Bibliography

Welcome to the research group of Prof. Dr. Cornelia M. Keck in Marburg. Cornelia M. Keck is a pharmacist and obtained her PhD in 2006 from the Freie Universität (FU) in Berlin. In 2009 she was appointed as Adjunct Professor for Pharmaceutical and Nutritional Nanotechnology at the University Putra Malaysia (UPM) and in 2011 she obtained her Venia legendi (Habilitation) at the Freie Universität Berlin and was appointed as a Professor for Pharmacology and Pharmaceutics at the University of Applied Sciences Kaiserslautern. Since 2016 she is Professor of Pharmaceutics and Biopharmaceutics at the Philipps-Universität Marburg. Her field of research is the development and characterization of innovative nanocarriers for improved delivery of poorly soluble actives for healthcare and cosmetics. Prof. Keck is executive board member of the German Association of Nanotechnology (Deutscher Verband Nanotechnologie), Vize-chairman of the unit „Dermocosmetics“ at the German Society of Dermopharmacy, active member in many pharmaceutical societies and member of the BfR Committee for Cosmetics at the Federal Institute for Risk Assessment (BfR).

Journal Highlights
Abbreviation: J App Pharm
doi: http://dx.doi.org/10.21065/19204159
Frequency: Annual 
Current Volume: 9 (2017)
Next scheduled volume: December, 2018 (Volume 10)
Back volumes: 1-9
Starting year: 2009
Nature: Online 
Submission: Online  
Language: English

Subject & Scope
  • Pharmaceutics
  • Physical Pharmacy 
  • Dosage Forms Science 
  • Pharmaceutical Microbiology & Immunology 
  • Industrial Pharmacy 
  • Bio-Pharmaceutics 
  • Pharmaceutical Chemistry 
  • Pharmaceutical Instrumentation 
  • Medicinal Chemistry 
  • Pharmacognosy 
  • Physiology &Histology 
  • Anatomy & Pathology 
  • Pharmacology & Therapeutics 
  • Pharmacy Practice 
  • Pharmaceutical Mathematics   
  • Biostatistics 
  • Dispensing 
  • Community Social & Administrative Pharmacy 
  • Hospital Pharmacy 
  • Clinical Pharmacy 
  • Pharmaceutical Quality Management 
  • Forensic Pharmacy 
  • Pharmaceutical Technology 
  • Pharmaceutical Management & Marketing

Consortium Publisher is an online publisher that enjoys global presence with International Journals

Follow Us

©2009 - 2019 Consortium Publisher Canada

Contact Info

6252 Lisgar Dr Mississauga Ontario L5N7V2 Canada
+1 (647) 526-0885
office@consortiumpublisher.ca
http://www.consortiumpublisher.ca/